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Fixed broken links (fixes #2)
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Renaud committed Mar 22, 2017
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2 changes: 1 addition & 1 deletion README.md
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Expand Up @@ -28,7 +28,7 @@ library(bseqsc)

## More information

* [Detailed installation instructions](http://shenorrlab.github.io/bseqsc/articles/pages/installation.html)
* [Detailed installation instructions](http://shenorrlab.github.io/bseqsc/vignettes/pages/installation.html)
* [Sample analysis](http://shenorrlab.github.io/bseqsc/vignettes/bseq-sc.html)
* [Documentation](http://shenorrlab.github.io/bseqsc/reference/index.html)

2 changes: 1 addition & 1 deletion _pkgdown.yaml
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Expand Up @@ -7,7 +7,7 @@ navbar:
- text: "Documentation"
href: "reference/index.html"
- dir: vignettes/pages
output: articles/pages
output: vignettes/pages
right:
- icon: fa-github
href: https://github.com/shenorrlab/bseq-sc
12 changes: 6 additions & 6 deletions vignettes/bseq-sc.Rmd
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Expand Up @@ -35,7 +35,7 @@ opts_chunk$set(dpi=300, dev="png")#, dev.args=list(pointsize=15))
IS_PKGDOWN <- nzchar(Sys.getenv('_R_PKGDOWN_RENDER'))
# packages
library(BiocStyle)
# pkgmaker::load_project(pancreas)
# pkgmaker::load_project('pancreas/bseq-sc')
library(bseqsc)
library(ggplot2)
library(reshape2)
Expand Down Expand Up @@ -70,7 +70,7 @@ When using this work in any way, please cite the following work:
## Software

In order to run the entire deconvolution pipeline, users need to install the `r Githubpkg('shenorrlab/bseq-sc', 'bseqsc')` R package from GitHub.
See the [installtion instructions](installation.html) for details.
See the [installtion instructions](pages/installation.html) for details.

## Data
`r BSEQSC` uses two types of input data:
Expand Down Expand Up @@ -109,15 +109,15 @@ Here we use two `ExpressionSet` objects to handle the bulk and single cell data
The dataset's [GEO entry (GSE50244)](http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE50244) contains raw RNA-seq and sample annotation data.
For the purpose of this vignette, we will use data that we pre-processed and
made available on the [data
download page](http://shenorrlab.github.io/BSeq-sc/data).
download page](pages/data.html).

After pre-processing, the data were filtered to remove unmapped tags, or tags that mapped to pseudogenes, or known ribosomal/mithocondrial genes (list available in file `data/genes_mitrib.txt.gz` or via function `getMITRIB()` provided in the package).
Moreover, counts from transcripts that mapped to a same gene were averaged.
Sample phenotypic annotations were also processed to define glycemic groups based on *hba1c* measurements, as per the original publication [@Fadista2014].

```{r, eval = !local_data}
# download GEO dataset from Github
eset <- readRDS('http://shenorrlab.github.io/BSeq-sc/data/GSE50244.rds')
eset <- readRDS('https://shenorrlab.github.io/bseqsc/data/GSE50244.rds')
```
```{r, eset}
# for this analysis we only look at samples with hba1c data
Expand All @@ -128,10 +128,10 @@ eset
### Single cell data
We generated single cell RNA-seq data of pancreatic islets from 3 healthy individuals, which provided gene expression profiles for 17434 genes in 7729 cells.
The raw counts for these data are available on the [data download
page](http://shenorrlab.github.io/BSeq-sc/data), in the form of an
page](pages/data.html), in the form of an
`ExpressionSet`:
```{r islet_eset, eval = !local_data}
eislet <- readRDS('http://shenorrlab.github.io/BSeq-sc/data/islet-eset.rds')
eislet <- readRDS('https://shenorrlab.github.io/bseqsc/data/islet-eset.rds')
eislet
```

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4 changes: 2 additions & 2 deletions vignettes/pages/data.Rmd
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Expand Up @@ -6,6 +6,6 @@ output:
---

* [Processed RNA-seq data from bulk pancreatic islets in healthy and
hyperglycemic individuals (GSE50244)](../data/GSE50244.rds)
hyperglycemic individuals (GSE50244)](../../data/GSE50244.rds)
* [Single cell RNA-seq data of pancreatic islets from healthy
individuals](../data/islet-eset.rds)
individuals](../../data/islet-eset.rds)
2 changes: 1 addition & 1 deletion vignettes/pages/installation.Rmd
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Expand Up @@ -14,7 +14,7 @@ installed from GitHub by:
install.packages('devtools')
# install bseqsc
devtools::install_github('shenorrlab/bseq-sc')
devtools::install_github('shenorrlab/bseqsc')
# load
library(bseqsc)
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