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Add metro map and update documentation to pass lint
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Vangelis Theodorakis committed Aug 23, 2023
1 parent a34e1d5 commit b1f6e20
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11 changes: 1 addition & 10 deletions .ipynb_checkpoints/README-checkpoint.md
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**buildrnaseqbundle** is a bioinformatics pipeline that can be used to create bundles of input data (genome files, annotations, indexes etc) for RNA sequencing analyses with **nf-core/rnaseq**, using the [guidelines for GTEx v10](https://github.com/broadinstitute/gtex-pipeline/blob/master/TOPMed_RNAseq_pipeline.md).

<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core
workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples. -->
<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->
[](buildrnaseqbundle-metro-map.drawio.png)

1. Get the input fasta and gtf files
1. Prepre the reference genome file
Expand All @@ -41,17 +39,10 @@
You can run the pipeline using:

<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->

```bash
nextflow run nf-core/buildrnaseqbundle \
-profile <docker/singularity/.../institute> \
--gencodeVersion "44" \
--spliceJunctionOverhang 100 \
--star_output_zip <STAR_OUTPUT_ZIP> \
--rsem_output_zip <RSEM_OUTPUT_ZIP> \
--executor <slurm/local>
--publish_intermediate_data <true/false>
--outdir <OUTDIR>
```

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67 changes: 67 additions & 0 deletions .ipynb_checkpoints/modules-checkpoint.json
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@@ -0,0 +1,67 @@
{
"name": "nf-core/buildrnaseqbundle",
"homePage": "https://github.com/nf-core/buildrnaseqbundle",
"repos": {
"https://github.com/nf-core/modules.git": {
"modules": {
"nf-core": {
"cat/cat": {
"branch": "master",
"git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
"installed_by": ["modules"]
},
"cat/fastq": {
"branch": "master",
"git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e",
"installed_by": ["modules"]
},
"custom/dumpsoftwareversions": {
"branch": "master",
"git_sha": "05c280924b6c768d484c7c443dad5e605c4ff4b4",
"installed_by": ["modules"]
},
"gatk4/createsequencedictionary": {
"branch": "master",
"git_sha": "541811d779026c5d395925895fa5ed35e7216cc0",
"installed_by": ["modules"]
},
"gunzip": {
"branch": "master",
"git_sha": "e06548bfa36ee31869b81041879dd6b3a83b1d57",
"installed_by": ["modules"]
},
"picard/createsequencedictionary": {
"branch": "master",
"git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
"installed_by": ["modules"]
},
"rsem/preparereference": {
"branch": "master",
"git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220",
"installed_by": ["modules"]
},
"samtools/faidx": {
"branch": "master",
"git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe",
"installed_by": ["modules"]
},
"star/genomegenerate": {
"branch": "master",
"git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220",
"installed_by": ["modules"]
},
"untar": {
"branch": "master",
"git_sha": "d0b4fc03af52a1cc8c6fb4493b921b57352b1dd8",
"installed_by": ["modules"]
},
"unzip": {
"branch": "master",
"git_sha": "cf67a6d7d043e2bd6a3099be84c72046fc71508f",
"installed_by": ["modules"]
}
}
}
}
}
}
6 changes: 3 additions & 3 deletions .ipynb_checkpoints/nextflow-checkpoint.config
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// Global default params, used in configs
params {
gencodeVersion = "44"
gencodeVersion = 44
spliceJunctionOverhang = 100 // Length of reads - 1
star_output_zip = "STARv2710a_genome_GRCh38_noALT_noHLA_noDecoy_ERCC_v44_oh100"
rsem_output_zip = "rsem_reference_GRCh38_gencode44_ercc"
executor = "slurm"
publish_intermediate_data = null
publish_intermediate_data = false

// Boilerplate options
input = null
outdir = "rnaseq-hg38-gencode.v44-bundle"
publish_dir_mode = 'copy'
input = null
email = null
email_on_fail = null
plaintext_email = false
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11 changes: 1 addition & 10 deletions README.md
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Expand Up @@ -14,9 +14,7 @@

**buildrnaseqbundle** is a bioinformatics pipeline that can be used to create bundles of input data (genome files, annotations, indexes etc) for RNA sequencing analyses with **nf-core/rnaseq**, using the [guidelines for GTEx v10](https://github.com/broadinstitute/gtex-pipeline/blob/master/TOPMed_RNAseq_pipeline.md).

<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core
workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples. -->
<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->
[](buildrnaseqbundle-metro-map.drawio.png)

1. Get the input fasta and gtf files
1. Prepre the reference genome file
Expand All @@ -41,17 +39,10 @@
You can run the pipeline using:

<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->

```bash
nextflow run nf-core/buildrnaseqbundle \
-profile <docker/singularity/.../institute> \
--gencodeVersion "44" \
--spliceJunctionOverhang 100 \
--star_output_zip <STAR_OUTPUT_ZIP> \
--rsem_output_zip <RSEM_OUTPUT_ZIP> \
--executor <slurm/local>
--publish_intermediate_data <true/false>
--outdir <OUTDIR>
```

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