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how do i generate the repeat sequences masked gtf file from Ensembl? #385

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erzakiev opened this issue Jan 12, 2024 · 0 comments
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@erzakiev
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I want to use ENSEMBL annotations because my CellRanger output was aligned using GRCh38 v 32 (Ensembl 98).

For that matter, I have the fasta sequence of the corresponding release, Homo_sapiens.GRCh38.dna_rm.toplevel.fa.gz, in which the repeat sequences were hard-masked by the Ensembl consortium themselves.

But to use it as an input for velocyto, I need to massage it into a gtf file. How should I do this?

@erzakiev erzakiev changed the title how do i generate the repeat sequences masked gtf files from Ensembl? how do i generate the repeat sequences masked gtf file from Ensembl? Jan 12, 2024
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