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I want to use ENSEMBL annotations because my CellRanger output was aligned using GRCh38 v 32 (Ensembl 98).
For that matter, I have the fasta sequence of the corresponding release, Homo_sapiens.GRCh38.dna_rm.toplevel.fa.gz, in which the repeat sequences were hard-masked by the Ensembl consortium themselves.
But to use it as an input for velocyto, I need to massage it into a gtf file. How should I do this?
The text was updated successfully, but these errors were encountered:
erzakiev
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how do i generate the repeat sequences masked gtf files from Ensembl?
how do i generate the repeat sequences masked gtf file from Ensembl?
Jan 12, 2024
I want to use ENSEMBL annotations because my CellRanger output was aligned using GRCh38 v 32 (Ensembl 98).
For that matter, I have the fasta sequence of the corresponding release, Homo_sapiens.GRCh38.dna_rm.toplevel.fa.gz, in which the repeat sequences were hard-masked by the Ensembl consortium themselves.
But to use it as an input for velocyto, I need to massage it into a gtf file. How should I do this?
The text was updated successfully, but these errors were encountered: