DNAWORKS.inp

# DNAWORKS.inp sample
# David Hoover, 2010-11-09
# 
# Directives must be flat against the left margin
#
# Comments demarcated by '#'.  All text following # is ignored.
#
# $I = integer
# $R = real, floating point number
# $S = string (must be in double quotes)
# [ ] = optional
# | = exclusive conditional
#
# title $S
# title "" # default
#  TITLE "mutant1"
#  TITLE "test2" 
#  TITLE "mutant2" 

# timelimit 0 # seconds until giving up, 0 means wait forever

# email $S
#  EMAIl "webtools@helix.nih.gov"

# melting low $I [ high $I ] [ tolerance $I ]
# melting low 62 # default
melting low 75 # default

# length low $I [ high $I ] [ random ]
# length low 40 # default
length low 180 # default

# frequency [ threshold $I ] [ random ] [ strict ] [ scored ]
# frequency threshold 10 # default

# concentration [ oligo $R ] [ sodium $R ] [ magnesium $R ]
# concentration oligo 1E-7 sodium 0.05 magnesium 0.002 # default

# solutions $I
# solutions 1 # default

# repeat $I
# repeat 8 # default

# misprime $I [ tip $I ] [ max $I ]
# misprime 18 tip 6 max 8 # default

# weight [ twt #R ] [ cwt #R ] [ rwt #R ] [ mwt #R ] [ gwt #R ] [ awt #R ] [ lwt #R ] [ pwt #R ] [ fwt #R ]
# weight twt 1.0 cwt 1.0 rwt 1.0 mwt 1.0 gwt 1.0 awt 1.0 lwt 1.0 pwt 1.0 fwt 1.0 # default

# tbio
# nogaps
# logfile $S
# logfile "LOGFILE.txt" # default
#  LOGFILE "MyOutput.txt"  
#  LOGFILE "mutant1.out"

# previous $I [ $S ]
# previous 1 "LOGFILE.txt" # default
#
# Mutant run:
#  PREVious 1
#  PREVious 1 "mutant1.out"
#

# pattern
#   AflII CTTAAG
#   BamHI GGATCC
# //

codon ecoli2

# protein #reverse #gapfix
protein
  pattdkslkd iliqgtknlp ileiasnnqp qnvdsvcsgt lqktedvhlm 
  gftlsgqkva dspleaskrw afrtgvppkn veytegeeak tcynisvtdp 
//

# nucleotide #reverse #gapfix
#   gagctcggat ccactactcg acccacgcgt ccgcccacgc gtccggccag gacctctgtg
#   aaccggtcgg ggcgggggcc gcctggccgg gagtctgctc ggcggtgggt ggccgaggaa
#   gggagagaac gatcgcggag cagggcgccc gaactccggg cgccgcgcca tgcgccgggc
#   cagccgagac tacggcaagt acctgcgcag ctcggaggag atgggcagcg gccccggcgt
#   cccacacgag ggtccgctgc accccgcgcc ttctgcaccg gctccggcgc cgccacccgc
#   cgcctcccgc tccatgttcc tggccctcct ggggctggga ctgggccagg tggtctgcag
#   catcgctctg ttcctgtact ttcgagcgca gatggatcct aacagaatat cagaagacag
#   cactcactgc ttttatagaa tcctgagact ccatgaaaac gcaggtttgc aggactcgac
#   tctggagagt gaagacacac tacctgactc ctgcaggagg atgaaacaag cctttcaggg
#   ggccgtgcag aaggaactgc aacacattgt ggggccacag cgcttctcag gagctccagc
#   tatgatggaa ggctcatggt tggatgtggc ccagcgaggc aagcctgagg cccagccatt
#   tgcacacctc accatcaatg ctgccagcat cccatcgggt tcccataaag tcactctgtc
#   ctcttggtac cacgatcgag gctgggccaa gatctctaac atgacgttaa gcaacggaaa
#   actaagggtt aaccaagatg gcttctatta cctgtacgcc aacatttgct ttcggcatca
#   tgaaacatcg ggaagcgtac ctacagacta tcttcagctg atggtgtatg tcgttaaaac
#   cagcatcaaa atcccaagtt ctcataacct gatgaaagga gggagcacga aaaactggtc
#   gggcaattct gaattccact tttattccat aaatgttggg ggatttttca agctccgagc
#   tggtgaagaa attagcattc aggtgtccaa cccttccctg ctggatccgg atcaagatgc
#   gacgtacttt ggggctttca aagttcagga catagactga gactcatttc gtggaacatt
# //
#
#
#
#-------------------------------------------------------------------------------
# OTHER EXAMPLES:
#-------------------------------------------------------------------------------

# Nucleotide examples:
#
# NUCLeotide
#   CCATG
# //
#
# NUCLeotide
#   GGGTTC
# //
#
# NUCLeotide
#    1 CCATGGCGGCTGGTCAGGCGTTCCGTAAATTCCTGCCGCTGTTCGACCGTGTTCTCGTGG
#   61 AACGCTCTGAAGTTGAAACC
# //
#
# NUCLeotide
#   RRW
# //
#
# NUCLeotide REVERSE
#   TCTGCGGGTGGTATCGTGCTGACCGGTTCTGCGGCTG
#  121 CGAAAGTGCTGCAGGCGACCGTTGTTGCGGTTGGTTCTGGTTCTAAAGGTAAAGGTGGT
# //
#
# NUCLeotide GAPFIX
#  NNN
# //
#
# NUCLeotide
# ATCCAGCCGGTTTCTGTTAAGGTTGGTGACAAAGTTCTGCTGCCGGAATACGGCGGTA
#  241 CCAAAGTTGTTCTGGACGACAAAGACTACTTCCTGTTCCGTGACGGTGACATCCTGGGTA
#  301 AGTACGTTGACTAAGGGTTC
# //
#
# Protein examples:
#
# PROTein
#   AAGQAFRKFLPLFDRVLVERSEVET
# //
#
# PROTein GAPFIX
#   K
# //
#
# PROTein
#   SAGGIVLTGSAAAKVLQATVVAVGSGSKGKGG
# //
#
# PROTein GAPFIX
#   E
# //
#
# PROTein
#   IQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVDX
# //
#
# Pattern examples:
#
# PATTern
#   EcoRI       GAATTC
#   PstI        CtgcaG
#   BamHI       GGATCC
#   KpnI        GGTACC
#    NdeI        CATATG
#    PvuII       CAGCTG
#    SwaI        ATTTAAAT
#    FseI        GGCCGGCC
#    NotI        GCGGCCGC
#    NcoI        CCATGG
#    silly   RWGGTcGRY  
# //
#
# Codon Frequency Tables:
#
# CODOn S. cerevesiae 
#
# CODOn E. coli 
#
# CODOn ecoli2 
#
# CODOn 
#Gly     GGG     40359.00     11.39      0.16
#Gly     GGA     34894.00      9.85      0.13
#Gly     GGT     89915.00     25.37      0.35
#Gly     GGC     94608.00     26.70      0.36
#Glu     GAG     66665.00     18.81      0.33
#Glu     GAA    137748.00     38.87      0.67
#Asp     GAT    116164.00     32.78      0.63
#Asp     GAC     67865.00     19.15      0.37
#Val     GTG     85263.00     24.06      0.34
#Val     GTA     41283.00     11.65      0.17
#Val     GTT     70627.00     19.93      0.29
#Val     GTC     50417.00     14.23      0.20
#Ala     GCG    104293.00     29.43      0.32
#Ala     GCA     75329.00     21.26      0.23
#Ala     GCT     60787.00     17.15      0.19
#Ala     GCC     85138.00     24.03      0.26
#Arg     AGG      7966.00      2.25      0.04
#Arg     AGA     13784.00      3.89      0.07
#Ser     AGT     35966.00     10.15      0.16
#Ser     AGC     53286.00     15.04      0.24
#Lys     AAG     45133.00     12.74      0.26
#Lys     AAA    125351.00     35.37      0.74
#Asn     AAT     75086.00     21.19      0.50
#Asn     AAC     75334.00     21.26      0.50
#Met     ATG     92952.00     26.23      1.00
#Ile     ATA     25982.00      7.33      0.12
#Ile     ATT    105218.00     29.69      0.49
#Ile     ATC     83118.00     23.46      0.39
#Thr     ACG     48560.00     13.70      0.25
#Thr     ACA     34483.00      9.73      0.17
#Thr     ACT     37430.00     10.56      0.19
#Thr     ACC     77023.00     21.74      0.39
#Trp     TGG     48949.00     13.81      1.00
#End     TGA      3616.00      1.02      0.31
#Cys     TGT     18601.00      5.25      0.46
#Cys     TGC     21434.00      6.05      0.54
#End     TAG       978.00      0.28      0.08
#End     TAA      7024.00      1.98      0.60
#Tyr     TAT     62750.00     17.71      0.59
#Tyr     TAC     43034.00     12.14      0.41
#Leu     TTG     45581.00     12.86      0.13
#Leu     TTA     51320.00     14.48      0.14
#Phe     TTT     78743.00     22.22      0.58
#Phe     TTC     56591.00     15.97      0.42
#Ser     TCG     29993.00      8.46      0.13
#Ser     TCA     32814.00      9.26      0.15
#Ser     TCT     37586.00     10.61      0.17
#Ser     TCC     32586.00      9.20      0.15
#Arg     CGG     21391.00      6.04      0.11
#Arg     CGA     13645.00      3.85      0.07
#Arg     CGT     70009.00     19.76      0.36
#Arg     CGC     68569.00     19.35      0.35
#Gln     CAG    100346.00     28.32      0.66
#Gln     CAA     51275.00     14.47      0.34
#His     CAT     44633.00     12.60      0.58
#His     CAC     32678.00      9.22      0.42
#Leu     CTG    168885.00     47.66      0.47
#Leu     CTA     15275.00      4.31      0.04
#Leu     CTT     42704.00     12.05      0.12
#Leu     CTC     35873.00     10.12      0.10
#Pro     CCG     72450.00     20.44      0.49
#Pro     CCA     30515.00      8.61      0.21
#Pro     CCT     26805.00      7.56      0.18
#Pro     CCC     19008.00      5.36      0.13
#//


#-------------------------------------------------------------------------------
# MORE EXAMPLES TO TRY:
#-------------------------------------------------------------------------------

#NUCLeotide 
#        1 gagctcggat ccactactcg acccacgcgt ccgcccacgc gtccggccag gacctctgtg
#       61 aaccggtcgg ggcgggggcc gcctggccgg gagtctgctc ggcggtgggt ggccgaggaa
#      121 gggagagaac gatcgcggag cagggcgccc gaactccggg cgccgcgcca tgcgccgggc
#      181 cagccgagac tacggcaagt acctgcgcag ctcggaggag atgggcagcg gccccggcgt
#      241 cccacacgag ggtccgctgc accccgcgcc ttctgcaccg gctccggcgc cgccacccgc
#      301 cgcctcccgc tccatgttcc tggccctcct ggggctggga ctgggccagg tggtctgcag
#      361 catcgctctg ttcctgtact ttcgagcgca gatggatcct aacagaatat cagaagacag
#      421 cactcactgc ttttatagaa tcctgagact ccatgaaaac gcaggtttgc aggactcgac
#      481 tctggagagt gaagacacac tacctgactc ctgcaggagg atgaaacaag cctttcaggg
#      541 ggccgtgcag aaggaactgc aacacattgt ggggccacag cgcttctcag gagctccagc
#      601 tatgatggaa ggctcatggt tggatgtggc ccagcgaggc aagcctgagg cccagccatt
#      661 tgcacacctc accatcaatg ctgccagcat cccatcgggt tcccataaag tcactctgtc
#      721 ctcttggtac cacgatcgag gctgggccaa gatctctaac atgacgttaa gcaacggaaa
#      781 actaagggtt aaccaagatg gcttctatta cctgtacgcc aacatttgct ttcggcatca
#      841 tgaaacatcg ggaagcgtac ctacagacta tcttcagctg atggtgtatg tcgttaaaac
#      901 cagcatcaaa atcccaagtt ctcataacct gatgaaagga gggagcacga aaaactggtc
#      961 gggcaattct gaattccact tttattccat aaatgttggg ggatttttca agctccgagc
#     1021 tggtgaagaa attagcattc aggtgtccaa cccttccctg ctggatccgg atcaagatgc
#     1081 gacgtacttt ggggctttca aagttcagga catagactga gactcatttc gtggaacatt
#//



#NUCLeotide REVERSE
#      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
#      421 actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
#//

#PROTein gapfix
#RRRRR
#//

#NUCLeotide
#GTAGCGACTAGCAT
#//

#NUCLeotide
#      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
#      421 actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
#//

#PROTein REVERSE (hBD2)
#  GIGDPVTCLDCGAISHPVFCPDRYKQIGTCGLPGTKCCKKPXX
#//

#NUCLeotide gapfix
#TGATGATTATTA
#//

#PROTein
#  KVFGDCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINS
#  RWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDV
#  XX
#//

#NUCLeotide gapfix
#TAGAAAACGC
#//


#PROTein (GFP)
#  1 mskgeelftg vvpilveldg dvnghkfsvs gegegdatyg kltlkfictt gklpvpwptl
# 61 vttfsygvqc fsrypdhmkq hdffksampe gyvqertiff kddgnyktra evkfegdtlv
#121 nrielkgidf kedgnilghk leynynshnv yimadkqkng ikvnfkirhn iedgsvqlad
#281 hyqqntpigd gpvllpdnhy lstqsalskd pnekrdhmvl lefvtaagit hgmdelyk
#//

#NUCLeotide (GFP)
#   1 GGGGGGGGGGGTGAAGAACTGTTCACCGGCGTTGTTCCGATCCTGGTTGAACTGGATGGT
#  61 GACGTGAATGGTCACAAATTCTCTGTTTCTGGTGAGGGTGAAGGCGACGCGACCTACGGC
# 121 AAACTCACCCTGAAATTCATCTGCACCACCGGTAAACTGCCGGTTCCGTGGCCGACCCTG
# 181 GTTACCACCTTCTCTTACGGTGTTCAGTGTTTCTCTCGTTATCCGGACCACATGAAACAG
# 241 CACGATTTTTTCAAATCTGCGATGCCGGAAGGTTACGTTCAGGAACGTACCATCTTCTTC
# 301 AAGGACGACGGCAACTATAAAACCCGTGCGGAAGTTAAATTCGAAGGTGACACCCTCGTG
# 361 AACCGTATCGAACTGAAAGGTATCGACTTCAAAGAAGACGGTAATATCCTGGGCCACAAA
# 421 CTCGAATACAACTACAACTCCCACAACGTTTACATTATGGCGGACAAGCAAAAGAACGGT
# 481 ATCAAAGTGAACTTCAAGATCCGCCACAACATCGAGGACGGTTCTGTTCAGCTCGCGGAT
# 541 CACTACCAACAGAATACCCCAATCGGCGACGGTCCGGTTCTCCTGCCGGACAACCACTAT
# 601 CTGTCTACCCAGTCTGCGCTGTCTAAGGACCCGAACGAAAAACGCGATCATATGGTGCTG
# 661 CTGGAATTCGTTACCGCGGCTGGTATTACTCACGGTATGGACGAACTGTACAAA
#//

#PROTein (Ovalbumin)
#  1 gsigaasmef cfdvfkelkv hhanenifyc piaimsalam vylgakdstr tqinkvvrfd
# 61 klpgfgdxie aqcgtsvnvh sslrdilnqi tkpndvysfs lasrlyaeer ypilpeylqc
#121 vkelyrggle pinfqtaadq arelinswve sqtngiirnv lqpxsvdsqt amvlvnaivf
#181 kglwekafkd edtqampfrv teqeskpvqm myqiglfrva smasekmkil elpfaxgtms
#241 mlvllpdevs gleqlesiin fekltewtss nvmeerkikv ylprmkmeek ynltsvlmam
#301 gitdvfsssa nlsgissaex lkisqavhaa haeineagre vvgxaeagvd aasvseefra
#361 dhpflfcikh iatnavlffg rcvsp
#//

#PROTein (Human Asparaginase) REVERSE
#  1 MAHHHHHHAR AVGPERRLLA VYTGGTIGMR SELGVLVPGT GLAAILRTLP MFHDEEHARA
# 61 RGLSEDTLVL PPDSRNQRIL YTVLECQPLF DSSDMTIAEW VRVAQTIKRH YEQYHGFVVI
#121 HGTDTMAFAA SMLSFMLENL QKTVILTGAQ VPIHALWSDG RENLLGALLM AGQYVIPEVC
#181 LFFQNQLFRG NRATKVDARR FAAFCSPNLL PLATVGADIT INRELVRKVD GKAGLVVHSS
#241 MEQDVGLLRL YPGIPAALVR AFLQPPLKGV VMETFGSGNG PTKPDLLQEL RVATERGLVI
#301 VNCTHCLQGA VTTDYAAGMA MAGAGVISGF DMTSEAALAK LSYVLGQPGL SLDVRKELLT
#361 KDLRGEMTPP SVEERRPSLQ GNTLGGGVSW LLSLSGSQEA DALRNALVPS LACAAAHAGD
#421 VEALQALVEL GSDLGLVDFN GQTPLHAAAR GGHTEAVTML LQRGVDVNTR DTDGFSPLLL
#481 AVRGRHPGVI GLLREAGASL STQELEEAGT ELCRLAYRAD LEGLQVWWQA GADLGQPGYD
#541 GHSALHVAEA AGNLAVVAFL QSLEGAVGAQ APCPEVLPGV X
#//

#PROTein (lysine ketoglutarate reductase/saccharopine dehydrogenase)
#  1 MLQVHRTGLG RLGVSLSKGL HHKAVLAVRR EDVNAWERRA PLAPKHIKGI TNLGYKVLIQ
# 61 PSNRRAIHDK DYVKAGGILQ EDISEACLIL GVKRPPEEKL MSRKTYAFFS HTIKAQEANM
#121 GLLDEILKQE IRLIDYEKMV DHRGVRVVAF GQWAGVAGMI NILHGMGLRL LALGHHTPFM
#181 HIGMAHNYRN SSQAVQAVRD AGYEISLGLM PKSIGPLTFV FTGTGNVSKG AQAIFNELPC
#241 EYVEPHELKE VSQTGDLRKV YGTVLSRHHH LVRKTDAVYD PAEYDKHPER YISRFNTDIA
#301 PYTTCLINGI YWEQNTPRLL TRQDAQSLLA PGKFSPAGVE GCPALPHKLV AICDISADTG
#361 GSIEFMTECT TIEHPFCMYD ADQHIIHDSV EGSGILMCSI DNLPAQLPIE ATECFGDMLY
#421 PYVEEMILSD ATQPLESQNF SPVVRDAVIT SNGTLPDKYK YIQTLRESRE RAQSLSMGTR
#481 RKVLVLGSGY ISEPVLEYLS RDGNIEITVG SDMKNQIEQL GKKYNINPVS MDICKQEEKL
#541 GFLVAKQDLV ISLLPYVLHP LVAKACITNK VNMVTASYIT PALKELEKSV EDAGITIIGE
#601 LGLDPGLDHM LAMETIDKAK EVGATIESYI SYCGGLPAPE HSNNPLRYKF SWSPVGVLMN
#661 VMQSATYLLD GKVVNVAGGI SFLDAVTSMD FFPGLNLEGY PNRDSTKYAE IYGISSAHTL
#721 LRGTLRYKGY MKALNGFVKL GLINREALPA FRPEANPLTW KQLLCDLVGI SPSSEHDVLK
#781 EAVLKKLGGD NTQLEAAEWL GLLGDEQVPQ AESILDALSK HLVMKLSYGP EEKDMIVMRD
#841 SFGIRHPSGH LEHKTIDLVA YGDINGFSAM AKTVGLPTAM AAKMLLDGEI GAKGLMGPFS
#901 KEIYGPILER IKAEGIIYTT QSTIKPX
#//

#NUCLeotide
#GGGG
#//

#NUCLeotide (pUC18)
#        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
#       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
#      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
#      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
#      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
#      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
#      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
#      421 actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
#      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
#      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
#      601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
#      661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
#      721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
#      781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
#      841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
#      901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
#      961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
#     1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
#     1081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
#     1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
#     1201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
#     1261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
#     1321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
#     1381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
#     1441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
#     1501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
#     1561 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
#     1621 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
#     1681 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
#     1741 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
#     1801 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
#     1861 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
#     1921 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
#     1981 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
#     2041 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
#     2101 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
#     2161 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
#     2221 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
#     2281 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
#     2341 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
#     2401 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
#     2461 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
#     2521 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
#     2581 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
#     2641 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
#//