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proposed chimeric reads #661

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ejc043 opened this issue Nov 20, 2024 · 0 comments
Open

proposed chimeric reads #661

ejc043 opened this issue Nov 20, 2024 · 0 comments

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@ejc043
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ejc043 commented Nov 20, 2024

Thank you for the wonderful and comprehensive tool.
It says that we should expect ~10% chimeric reads and an abnormal %chimeric reads might suggest issues with library prep.However, typical hic kits like arima seem to enrich for crosslinked genomic regions and thus shouldn't we observe a higher % of chimeric reads? Or am I missing something?
I ask because I'm observing about 40-50% chimeric reads.
Thank you so much,
Eunice

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