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Their counts in the samples differ slightly though. I would expect these isomiRs to be merged and counted together in the output. I understand from mirtop.gff that they come from different precursors with identical mature sequences, but then I don't understand why their counts are different in the same sample?
Is this a bug? If not, what is the reason behind repeated isomiRs with identical sequences but different counts in the same sample?
The text was updated successfully, but these errors were encountered:
bounlu
changed the title
Repeated iromiRs in the mirtop.tsv output
Repeated isomiRs in the mirtop.tsv output
May 2, 2024
Thanks for this, I think it would be good to see more. I see here there are 3 sequences, and 2 are annotated for 199a and another to 199b. Probably 199a has two precursors. Can you share as well the file where you say the counts are different? Are you referring to the isomiR counts or to the miRNA counts?
The
mirtop.tsv
output contains repeated rows for some isomiRs with identical first 12 columns:Their counts in the samples differ slightly though. I would expect these isomiRs to be merged and counted together in the output. I understand from
mirtop.gff
that they come from different precursors with identical mature sequences, but then I don't understand why their counts are different in the same sample?Is this a bug? If not, what is the reason behind repeated isomiRs with identical sequences but different counts in the same sample?
The text was updated successfully, but these errors were encountered: