diff --git a/CHANGELOG.md b/CHANGELOG.md
index 746172c7f036..52ff51bd42d0 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -14,6 +14,7 @@
 - `[jest-core]` make `TestWatcher` extend `emittery` ([#10324](https://github.com/facebook/jest/pull/10324))
 - `[jest-core]` Run failed tests interactively the same way we do with snapshots ([#10858](https://github.com/facebook/jest/pull/10858))
 - `[jest-core]` more `TestSequencer` methods can be async ([#10980](https://github.com/facebook/jest/pull/10980))
+- `[jest-core]` Add support for `testSequencer` written in ESM ([#11207](https://github.com/facebook/jest/pull/11207))
 - `[jest-environment-node]` Add AbortController to globals ([#11182](https://github.com/facebook/jest/pull/11182))
 - `[@jest/fake-timers]` Update to `@sinonjs/fake-timers` to v7 ([#11198](https://github.com/facebook/jest/pull/11198))
 - `[jest-haste-map]` Handle injected scm clocks ([#10966](https://github.com/facebook/jest/pull/10966))
diff --git a/e2e/__tests__/customEsmTestSequencers.test.ts b/e2e/__tests__/customEsmTestSequencers.test.ts
new file mode 100644
index 000000000000..6fdadb280af8
--- /dev/null
+++ b/e2e/__tests__/customEsmTestSequencers.test.ts
@@ -0,0 +1,32 @@
+/**
+ * Copyright (c) Facebook, Inc. and its affiliates. All Rights Reserved.
+ *
+ * This source code is licensed under the MIT license found in the
+ * LICENSE file in the root directory of this source tree.
+ */
+
+import * as path from 'path';
+import {onNodeVersions} from '@jest/test-utils';
+import {extractSummary} from '../Utils';
+import runJest from '../runJest';
+const dir = path.resolve(__dirname, '../custom-esm-test-sequencer');
+
+onNodeVersions('^12.16.0 || >=13.7.0', () => {
+  test('run prioritySequence', () => {
+    const result = runJest(dir, ['-i'], {
+      nodeOptions: '--experimental-vm-modules --no-warnings',
+    });
+
+    expect(result.exitCode).toBe(0);
+    const sequence = extractSummary(result.stderr)
+      .rest.replace(/PASS /g, '')
+      .split('\n');
+    expect(sequence).toEqual([
+      './a.test.js',
+      './b.test.js',
+      './c.test.js',
+      './d.test.js',
+      './e.test.js',
+    ]);
+  });
+});
diff --git a/e2e/custom-esm-test-sequencer/a.test.js b/e2e/custom-esm-test-sequencer/a.test.js
new file mode 100644
index 000000000000..1187e8309f87
--- /dev/null
+++ b/e2e/custom-esm-test-sequencer/a.test.js
@@ -0,0 +1,7 @@
+/**
+ * Copyright (c) Facebook, Inc. and its affiliates. All Rights Reserved.
+ *
+ * This source code is licensed under the MIT license found in the
+ * LICENSE file in the root directory of this source tree.
+ */
+test('a', () => {});
diff --git a/e2e/custom-esm-test-sequencer/b.test.js b/e2e/custom-esm-test-sequencer/b.test.js
new file mode 100644
index 000000000000..04e77e777bb1
--- /dev/null
+++ b/e2e/custom-esm-test-sequencer/b.test.js
@@ -0,0 +1,7 @@
+/**
+ * Copyright (c) Facebook, Inc. and its affiliates. All Rights Reserved.
+ *
+ * This source code is licensed under the MIT license found in the
+ * LICENSE file in the root directory of this source tree.
+ */
+test('b', () => {});
diff --git a/e2e/custom-esm-test-sequencer/c.test.js b/e2e/custom-esm-test-sequencer/c.test.js
new file mode 100644
index 000000000000..332d8682ab00
--- /dev/null
+++ b/e2e/custom-esm-test-sequencer/c.test.js
@@ -0,0 +1,7 @@
+/**
+ * Copyright (c) Facebook, Inc. and its affiliates. All Rights Reserved.
+ *
+ * This source code is licensed under the MIT license found in the
+ * LICENSE file in the root directory of this source tree.
+ */
+test('c', () => {});
diff --git a/e2e/custom-esm-test-sequencer/d.test.js b/e2e/custom-esm-test-sequencer/d.test.js
new file mode 100644
index 000000000000..4102fe8c1925
--- /dev/null
+++ b/e2e/custom-esm-test-sequencer/d.test.js
@@ -0,0 +1,7 @@
+/**
+ * Copyright (c) Facebook, Inc. and its affiliates. All Rights Reserved.
+ *
+ * This source code is licensed under the MIT license found in the
+ * LICENSE file in the root directory of this source tree.
+ */
+test('d', () => {});
diff --git a/e2e/custom-esm-test-sequencer/e.test.js b/e2e/custom-esm-test-sequencer/e.test.js
new file mode 100644
index 000000000000..6b4bbf07b9b4
--- /dev/null
+++ b/e2e/custom-esm-test-sequencer/e.test.js
@@ -0,0 +1,7 @@
+/**
+ * Copyright (c) Facebook, Inc. and its affiliates. All Rights Reserved.
+ *
+ * This source code is licensed under the MIT license found in the
+ * LICENSE file in the root directory of this source tree.
+ */
+test('e', () => {});
diff --git a/e2e/custom-esm-test-sequencer/package.json b/e2e/custom-esm-test-sequencer/package.json
new file mode 100644
index 000000000000..4bc9288b13b7
--- /dev/null
+++ b/e2e/custom-esm-test-sequencer/package.json
@@ -0,0 +1,8 @@
+{
+  "type": "module",
+  "jest": {
+    "testEnvironment": "node",
+    "transform": {},
+    "testSequencer": "<rootDir>/testSequencer.mjs"
+  }
+}
diff --git a/e2e/custom-esm-test-sequencer/testSequencer.mjs b/e2e/custom-esm-test-sequencer/testSequencer.mjs
new file mode 100644
index 000000000000..f01adee77e64
--- /dev/null
+++ b/e2e/custom-esm-test-sequencer/testSequencer.mjs
@@ -0,0 +1,15 @@
+/**
+ * Copyright (c) Facebook, Inc. and its affiliates. All Rights Reserved.
+ *
+ * This source code is licensed under the MIT license found in the
+ * LICENSE file in the root directory of this source tree.
+ */
+
+import Sequencer from '@jest/test-sequencer';
+
+export default class CustomSequencer extends Sequencer.default {
+  sort(tests) {
+    const copyTests = Array.from(tests);
+    return copyTests.sort((testA, testB) => (testA.path > testB.path ? 1 : -1));
+  }
+}
diff --git a/packages/jest-core/src/runJest.ts b/packages/jest-core/src/runJest.ts
index e54a2435a77e..008f102a4a4e 100644
--- a/packages/jest-core/src/runJest.ts
+++ b/packages/jest-core/src/runJest.ts
@@ -20,7 +20,7 @@ import type {Config} from '@jest/types';
 import type {ChangedFiles, ChangedFilesPromise} from 'jest-changed-files';
 import type {Test} from 'jest-runner';
 import type {Context} from 'jest-runtime';
-import {interopRequireDefault, tryRealpath} from 'jest-util';
+import {requireOrImportModule, tryRealpath} from 'jest-util';
 import {JestHook, JestHookEmitter} from 'jest-watcher';
 import type FailedTestsCache from './FailedTestsCache';
 import SearchSource from './SearchSource';
@@ -142,9 +142,9 @@ export default async function runJest({
   failedTestsCache?: FailedTestsCache;
   filter?: Filter;
 }): Promise<void> {
-  const Sequencer: typeof TestSequencer = interopRequireDefault(
-    require(globalConfig.testSequencer),
-  ).default;
+  const Sequencer: typeof TestSequencer = await requireOrImportModule(
+    globalConfig.testSequencer,
+  );
   const sequencer = new Sequencer();
   let allTests: Array<Test> = [];