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I got errors when running "RcisTarget::addSignificantGenes" by following the tutorial posted on "https://www.bioconductor.org/packages/release/bioc/vignettes/RcisTarget/inst/doc/RcisTarget_MainTutorial.html#Running_RcisTarget". my script (and the results not the same as the tutorial):
txtFile <- paste(file.path(system.file('examples', package='RcisTarget')),
"hypoxiaGeneSet.txt", sep="/")
geneLists <- list(hypoxia=read.table(txtFile, stringsAsFactors=FALSE)[,1])
head(geneLists$hypoxia)
motifRankings <- importRankings("mc_v10_clust/gene_based/hg38_10kbp_up_10kbp_down_full_tx_v10_clust.genes_vs_motifs.rankings.feather")
data(motifAnnotations_hgnc)
motifAnnotations_hgnc[199:202,]
(my results is the same as shown in the tutorial)
head(motifEnrichmentTable[,-"TF_lowConf", with=FALSE]) (not the same as tutorial)
geneSet
motif
NES
AUC
highlightedTFs
TFinDB
TF_highConf
hypoxia
23459
4.13
0.0667
HIF1A, EPAS1
hypoxia
19265
3.91
0.0643
HIF1A, EPAS1
hypoxia
10830
3.58
0.0608
HIF1A, EPAS1
hypoxia
10469
3.42
0.0591
HIF1A, EPAS1
hypoxia
18527
3.41
0.0590
HIF1A, EPAS1
hypoxia
19969
3.38
0.0586
HIF1A, EPAS1
6 rows
follow the solution suggested by "jdenavascues" (Thanks a lot!!)
motifRankings@rankings <- dplyr::relocate(motifRankings@rankings, motifs)
and then it still did not go through.
motifEnrichmentTable_wGenes <- addSignificantGenes(motifEnrichmentTable,
geneSets=geneLists,
rankings=motifRankings,
nCores=1,
method="iCisTarget")
[1] 27090
Error in rccs[, sr] : subscript out of bounds
I have noticed that some users are having trouble with RcisTarget, especially regarding the different versions of the database.
Does anyone figure these out?
Y
The text was updated successfully, but these errors were encountered:
Hi RcisTarget,
I got errors when running "RcisTarget::addSignificantGenes" by following the tutorial posted on "https://www.bioconductor.org/packages/release/bioc/vignettes/RcisTarget/inst/doc/RcisTarget_MainTutorial.html#Running_RcisTarget".
my script (and the results not the same as the tutorial):
txtFile <- paste(file.path(system.file('examples', package='RcisTarget')),
"hypoxiaGeneSet.txt", sep="/")
geneLists <- list(hypoxia=read.table(txtFile, stringsAsFactors=FALSE)[,1])
head(geneLists$hypoxia)
motifRankings <- importRankings("mc_v10_clust/gene_based/hg38_10kbp_up_10kbp_down_full_tx_v10_clust.genes_vs_motifs.rankings.feather")
data(motifAnnotations_hgnc)
motifAnnotations_hgnc[199:202,]
(my results is the same as shown in the tutorial)
###############################
motifs_AUC <- calcAUC(geneLists, motifRankings, nCores=1)
motifEnrichmentTable <- addMotifAnnotation(
motifs_AUC,
nesThreshold=3,
motifAnnot=motifAnnotations_hgnc,
highlightTFs=list(hypoxia=c("HIF1A", "EPAS1")))
class(motifEnrichmentTable)
[1] "data.table" "data.frame"
dim(motifEnrichmentTable)
[1] 24 8
head(motifEnrichmentTable[,-"TF_lowConf", with=FALSE]) (not the same as tutorial)
follow the solution suggested by "jdenavascues" (Thanks a lot!!)
motifRankings@rankings <- dplyr::relocate(motifRankings@rankings, motifs)
and then it still did not go through.
motifEnrichmentTable_wGenes <- addSignificantGenes(motifEnrichmentTable,
geneSets=geneLists,
rankings=motifRankings,
nCores=1,
method="iCisTarget")
[1] 27090
Error in rccs[, sr] : subscript out of bounds
I have noticed that some users are having trouble with RcisTarget, especially regarding the different versions of the database.
Does anyone figure these out?
Y
The text was updated successfully, but these errors were encountered: